RNA World Informatics Phylogeny Database

NON-CANONICAL BASE PAIR DATABASE : Individual Record Page 422

Authors:	Agalarov SC, Sridhar Prasad G, Funke PM, Stout CD, Williamson JR
Citation:	Structure of the S15,S6,S18-rRNA complex: assembly of the 30S ribosome central domain Science 288:107-112 April 07, 2000
Base Pair Type:	GGC amino-carbonyl(G), N3-amino(C); Watson-Crick
Base Pair Category:	Triple Base Interactions; Major groove triple
Sequence Context:	5' U G(752) A 3' 5' A G(654) A 3' 5' A C(754) G 3'
Structure:	16S rRNA of T. thermophilus, Tth T4 RNA, helices 22 & 20
Pucker Info:	C754 & G752 both have the C2'endo conformation
Melting Point:	*
Free Energy:	*
Chemical Shift:	*
Glycosidic Conf:	C754 adopts syn conformation
Extra Info:	"This triple-base interaction occurs between the junction nucleotides G654 & G752 of helix 22 and C754 of helix 20. C754 is above the U652-A753 base pair and places helix 20 at an acute angle relative to helix 22. The C2'endo conformation of C754 & G752 creates a sharp bend in the RNA backbone between G752 and C754. C754 adopts the syn conformation and forms a Watson-Crick base pair with G654, and together they hydrogen bond with G752 forming a triple-base pair.
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