RNA World Informatics Phylogeny Database

NON-CANONICAL BASE PAIR DATABASE : Individual Record Page 431

Authors:	Anderson AC, O'Neil RH, Filman DJ, Frederick CA
Citation:	Crystal structure of a brominated RNA helix with four mismatched base pairs: An investigation into RNA conformational variability. Biochemistry 38(39):12577-85 September 28, 1999  [PDB link]
Base Pair Type:	UC 4-carbonyl-amino
Base Pair Category:	Normal Purine-Purine and Pyrimidine-Pyrimidine Base Pairs; Trans Watson-Crick/Watson-Crick
Sequence Context:	5'  3' U-G,Wobble U-C C-U,4-carbonyl-amino (1 bond) 3'  5'
Structure:	A brominated 15-nucleotide RNA helix
Pucker Info:	*
Melting Point:	*
Free Energy:	*
Chemical Shift:	*
Glycosidic Conf:	*
Extra Info:	C-U mismatched base pairs are the least thermodynamically stable of the mismatched pairs in the brominated helix, due to poor stacking interactions together with the presence of only a single hydrogen bond between the nucleotides. The C-U mismatched base pairs distort the A-form helix only slightly.
Figure: